Composite
Part:BBa_K2477013:Design
Designed by: Asha Kodan, Annie Trang, Cynthia Uzoukwu Group: iGEM17_UMaryland (2017-10-26)
Copper Biosensor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1237
Illegal AgeI site found at 1349 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Parts of this had to be ordered as a G-block and assembled via Gibson Assembly.
Source
The CueR and PcopA sequences were taken from E. coli. CueR was codon optimized. All other parts came from the iGEM kit.